Benzylaminopurine-induced coupling between calmodulin and Ca-ATPase in wheat root microsomal membranes

Calcium entry-calcium refilling (CECR) coupling between store-operated Ca(2+) entry and sarco/endoplasmic reticulum Ca(2+)-ATPase.

Cross-talk between subcellular organelles is essential for cellular Ca(2+) homeostasis. We have studied the effects of knocking down STIM1, the Ca(2+) sensor of the endoplasmic reticulum (ER), on several homeostatic Ca(2+)-handling mechanisms, including plasma membrane Ca(2+) entry and transport by ER, mitochondria and nucleus. We have used targeted aequorins to selectively measure calcium flux...

Calcium- and calmodulin-regulated breakdown of phospholipid by microsomal membranes from bean cotyledons.

Evidence for the involvement of Ca(2+) and calmodulin in the regulation of phospholipid breakdown by microsomal membranes from bean cotyledons has been obtained by following the formation of radiolabeled degradation products from [U-(14)C]phosphatidylcholine. Three membrane-associated enzymes were found to mediate the breakdown of [U-(14)C] phosphatidylcholine, viz. phospholipase D (EC 3.1.4.4)...

Calmodulin-stimulated Ca(2+)-ATPases in the vacuolar and plasma membranes in cauliflower.

The subcellular locations of Ca(2+)-ATPases in the membranes of cauliflower (Brassica oleracea L.) inflorescences were investigated. After continuous sucrose gradient centrifugation a 111-kD calmodulin (CaM)-stimulated and caM-binding Ca(2+)-ATPase (BCA1; P. Askerlund [1996] Plant Physiol 110: 913-922; S. Malmström, P. Askerlund, M.G. Plamgren [1997] FEBS Lett 400: 324-328) comigrated with vacu...

Ca-Calmodulin Promotes Survival of Pheromone-Induced Growth Arrest by Activation of Calcineurin and Ca-Calmodulin-Dependent Protein Kinase

Effects of Heavy Metal on Rat Liver Microsomal Ca”-ATPase and Ca2+ Sequestering

In isolated hepatic microsomal vesicles the heavy metals Cd*+, Cu’+, and Zn2+ inhibit Ca2+ uptake and evoke a prompt efflux of Ca2+ from preloaded vesicles in a dose-dependent manner. N-Ethylmaleimide also inhibits Ca2+ uptake and causes Ca2+ release, but it is less effective in these respects than the heavy metals. Measurement of mannose-6-phosphatase activity indicate that the heavy metal-ind...